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How repeatable is this? If you want to pursue it, grab a utility that calculates the MD5 or SHA-1 hash of files that you point it to; there are several free tools that do this. If you can create another one that’s broken in your main data store, calculate the hash of that file and compare it to the one that works to see if the files are in fact different. If they are, try copying both files to your local system and see if either hash changes — if one does, there was an issue reading from the original data store.
Finally, try initially saving the newly generated ISO file on your local system, check the hash there (and verify it works), then copy it to the data store and see if the hash changes.